In the present study defending potential of three indigenous plants assessed and compared using sperm abnormality nassay. Lagenaria siceraria, Desmodium gangeticum and Leucas aspera were selected on the basis of reported cytoprotective and antioxidant activity. Activity was evaluated using sperm abnormality assay. The animals were divided into four groups, four animals per group. The group distribution was, negative control, positive control (cyclophosphamide treated) and two treatment control group. Sperm abnormality was induced by administering cyclophosphamide (20 mg/kg, i.p) 24 hrs prior to sacrifice animals. Parameters selected are hook cut, banana shaped, amorphous shaped, double tailed and folded on them to execute sperm abnormality. The methanolic extract of selected indigenous plants had shown significant reduction in the total number of sperm abnormality. The results obtained by these experiments, strongly suggesting that Lagenaria siceraria and Desmodium gangeticum plants extract having potent while Leucas aspera plant extract shows the moderately defending property. Therefore, from the present study, it can be concluded that methanolic extract of selected indigenous plant extract possesses defending property. The observed defending activity of selected indigenous plant extracts against cyclophosphamide might be associated with active compounds and its antioxidant constituents such as poly-phenolic compound, flavonoids and other micronutrients.
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